Cytokine production
MLRs were applied in the combinations as described above. On day 5, supernatants from each well (100 μL) of selected samples (n = 13) were collected and frozen. The concentration of cytokines in each supernatant sample was analyzed in batches after thawing using a custom designed Luminex plate as per the manufacturer’s instructions (Bio-Techne, Minneapolis, MI, USA). The concentration of IFN-γ, IL-4, IL-6, IL-8, IL-10, IL-17 and TNF-α were analyzed using a Bio-Plex 200 plate reader and the Bio-Plex Manager 6.1 software (Bio-Rad, Hercules, CA, USA).