Tear N-glycosylation of AKC patients
All tears samples from the 7 AKC patients showed similar glyco-profiles (Figure 1C). Compared to CTRL and VKC, AKC spectra showed a strong increase of the species at m/z 2431.2 and 2792.4 (corresponding to biantennary N- glycans carrying one and two sialic acids respectively) and also a decrease of the ions at m/z 2605.3 and m/z 2966.5 associated to their fucosylated counterparts. Minor increase of the N- glycans corresponding to the sialylated triantennary series (m/z 3602.8 and m/z 3776.9) was detected. Furthermore, significant increase of oligomannose and bisected species besides a decrease of sialylated species, with the exception of the peak atm/z 2953.5, were observed (Figure 3B).