Experimental Design
On the basis of many studies, target group size (n ) ≥ 5 were
calculated (Portero et al., 2017; T. Yang et al., 2014). The number of
small dishes used in each group is at least 3 independent dishes. The
exact group size (n ) for each experimental group is found in
supplementary material online.
The study did not contain clinical studies or patient data or animal
experiments. hiPSC-CMs were randomised for treatment. In this study,
hiPSC-CMs were incubated with various concentrations of E2 (0.01, 1 and
100 nmol/L, 1 h). The stress model of hiPSC-CMs was pre-treated with E2
(0.01, 1 and 100 nmol/L, 30 min prior to ISO). hiPSC-CMs were treated
with G1 (11 nmol/L, 30 min) or G15 (20 nmol/L, 30 min) and small
interfering RNA.