Experimental Design
On the basis of many studies, target group size (n ) ≥ 5 were calculated (Portero et al., 2017; T. Yang et al., 2014). The number of small dishes used in each group is at least 3 independent dishes. The exact group size (n ) for each experimental group is found in supplementary material online.
The study did not contain clinical studies or patient data or animal experiments. hiPSC-CMs were randomised for treatment. In this study, hiPSC-CMs were incubated with various concentrations of E2 (0.01, 1 and 100 nmol/L, 1 h). The stress model of hiPSC-CMs was pre-treated with E2 (0.01, 1 and 100 nmol/L, 30 min prior to ISO). hiPSC-CMs were treated with G1 (11 nmol/L, 30 min) or G15 (20 nmol/L, 30 min) and small interfering RNA.