Data and Statistical Analysis
The data and statistical analysis comply with the recommendations on
experimental design and analysis in pharmacology (Curtis et al., 2018).
GraphPad Prism, Clampfit, Origin 2017, and ImageJ software were used for
data analysis. Data are shown as Mean ± S.E.M. Mean difference between
groups were compared with Unpaired Student’s t -test and One-way
ANOVA. Unpaired t -test with Welch’s correction and Kruskal-Wallis
test were used selectively in the case of uneven variance. Pearson
Correlation Coefficient was conducted to determine the correlation
between content of Nav1.5 and sodium current.P <0.05 was considered significant.
Results
Estrogen reduced the
ISO-induced increase in INaL
hiPSC-CMs were incubated with
different concentrations of E2 (0.01 nmol/L, 1 nmol/L and 100 nmol/L,
1h) to dissect estrogenic effects on cardiacI NaL. Results showed that E2 at any concentration
did not affect the average I NaL density in
hiPSC-CMs (Figure 2A and B). ISO treatment significantly elevatedI NaL density (Figure 1A and Figure 2A), but
pre-treatment with 1 nmol/L E2 abated the increase (Figure 2A and C).
Detailed data on Mean ± S.E.M and the number of cells are found in
supplementary material online, Supplemental Table 1.