GPER silencing by small interfering RNA
According to the procedure given by GenePharma (Suzhou, China), the transfection complex solution containing 16.7 nmol/L siRNA (GenePharma, China) was prepared, and then co-cultured with hiPSC-CMs serum-free medium at 37℃ for 4-6 hours. After that, the culture medium was replaced with serum-free medium and then cultured overnight prior to experimentation. Detail in GPER gene silencing sequence:
sense (5’-3’) : GGCCUCAUCUGGAUGGCAUTT;
antisense (5’-3’) : AUGCCAUCCAGAUGAGGCCTT.