Clamping Experiment
We tested the impacts of repeated clamping on leaves of Capsicum
annuum , Populus deltoides , and Populus trichocarpa . For
each of five sequential clamps on each measured leaf, we allowed the
chamber to stabilize (< 2 min in all cases), and measuredAnet at 400 μmol CO2mol-1 for 30 s at 0.5 Hz. Between clamps, the leaf was
given a 1 min recovery. On clamps 1 and 5, a RACiR was run as specified
above for each species. We then fit Vcmax andJmax as above. These measurements were performed
on an individual leaf spot within 30 min to minimize diurnal effects.