- Treatment Protocol
The patients with NMIBC received weekly intravesical BCG for six weeks, at least two weeks after the last TUR session. In each immunotherapy, 40 mg/ml of SII- Onco BCG (Serum Institute of India, Pune, India) was dissolved with 40 cc of isotonic NaCl and administered through a 12-Fr urethral catheter. This solution was retained in the bladder for 2 hours, after which urine collection was performed.
In the process of obtaining urine samples from each patient, the first visit was organized immediately before the first BCG instillation, the second visit immediately after the third instillation, the third visit immediately after the sixth instillation, and the last visit four weeks after the sixth instillation. In healthy participants, urine samples collection was performed at a single visit after the exclusion of bladder cancer and urinary tract infection using ultrasonography and urinalysis.