Metabolic and macroscopic evaluation during ESHP
The arterial pH was measured during the seven hours of reperfusion. With the aggressive management of sodium bicarbonate and CO2, pH remained relatively stable during this time period (Fig. 4a). As seen in Fig. 4b, lactate concentration increased continuously during SAM and PAM. Lactate concentration (in mmol/L) was 1.15 ± 0.49, 2.77 ± 0.18 and 2.90 ± 0.41 at SAM1, PAM and SAM2 respectively. Arterial and venous lactate concentration measurements were used to calculate the lactate extraction (Δlactate) at the different time points. The Δlactate (in mmol/L) between the venous and arterial perfusate samples was -0.01 ± 0.1, -0.06 ± 0.17, -0.12 ± 0.21 at SAM1, PAM and SAM2 respectively (Fig. 4c). As seen in table 2, myocardial weight increased during the experiment.