Ang II and Ang-(1–7) measurements
RV and lung supernatants were acidified with 0.6% trifluoroacetic acid
(TFA) to obtain a 10% (w/v) homogenate. The samples were centrifuged at
2,000 × g for 15 min at 4°C, and dried under a steam of nitrogen at
60°C. Enzyme-immunoassay kits (Phoenix Pharmaceuticals, CA, USA) were
used to measure the Ang II and Ang-(1–7) contents in the RV and lung.