Investigation of penetratin uptake by confocal microscopy
Penetratin uptake and endo-lysosomal release was visualized using a
Zeiss LSM880 confocal microscope (Carl Zeiss, Oberkochen, Germany).
Fluorescently labeled penetratins were used at the same concentration as
in flow cytometry. The temperature of the microscope stage was adjusted
to 37°C. AFDye532 and NF were excited at 514 nm and 633 nm,
respectively. Detection of AFDye532 and NF fluorescence was carried out
between 525-629 nm and 637-759 nm, respectively.