Cytokines
Cytokine concentrations in the serum were measured by ELISA as previously described (Chang et al., 2015; Jones, Ackermann, Wille, Hunter, & Scott, 2002). Purified anti-mouse capture and biotinylated detection of Abs to IL-12p70, IFN-γ, IL-4 were conducted. All Abs were obtained from BD Biosciences.