Flow cytometry
Splenocytes were incubated for 15 min with an anti-CD16/32 monoclonal antibody (mAb) to prevent the nonspecific binding of antibodies and were then reacted with primary mAb on ice for 30 min. The following mAbs were used in this study: FITC-conjugated anti-MHCII, PE-conjugated anti-CD80/86, and PE-Cy7 conjugated anti-F4/80. All flow cytometry data were acquired and analyzed with a FACS Calibur flow cytometer.