2.7 SFTSV whole genome sequencing and analysis
The PCR products were subjected to agarose gel electrophoresis, and the
amplified products were sent to Sangon Biotech (Shanghai) Co., Ltd for
sequencing. The sequences were spliced using the SeqMan software.
GenBank was used as source of reported strain sequences, and DNASTAR and
MEGA6.0 were used to conduct a comprehensive analysis of the three
fragments of the SFTSV strain, to identify the virus genotype, and to
analyze the genetic evolution of the strain. For paired and multiple
alignments, the maximum likelihood method (ML) was used to build a
phylogenetic tree based on the L, M, and S sequences. The bootstrap
method with 1000 replications was used to estimate the reliability of
the branches.