2.6 Amplification of SFTSV whole genome
Previously designed primers for
amplification of S (3 primer sets), M (7 primer sets), and L (13 primer
sets) segments were used for whole genome sequencing. Primers were
synthesized by Sangon Biotech (Shanghai) Co., Ltd. The size of the
target band amplified by each primer set for the S and M fragments was
around 600 bp. Amplification conditions: 94 ℃, 5 min; 35 cycles: 94 ℃,
30 s; 55 ℃, 30 s; 72 ℃ 1 min; 72 ℃, 5 min; 30-µL system. The size of the
target band amplified by each primer set for the L fragment was about
350‒650 bp. Amplification conditions: 94 ℃, 5 min; 35 cycles: 94 ℃, 30
s; 63 ℃, 30 s; 72 ℃, 1 min; 72 ℃, 5 min; 30-µL system.