From 2009-2013
For HHV6, in-house PCR used primers and Taqman probe (Applied Biosystems, Foster City, CA, USA) targeted at the U67 gene of HHV6, and the viral load threshold of detection was 233 copies/mL plasma at 95% confidence level. For EBV, in-house quantitative real-time PCR was targeted at the conserved region of EBNA-1 gene of EBV, and the viral load threshold of detection was 715 copies/mL plasma at 95% confidence level. For CMV, In-house quantitative real-time PCR with primers and probe were targeted at glycoprotein B-specific gene of human cytomegalovirus (CMV), and the viral load threshold of detection was 154 copies/mL plasma at 95% confidence level.