Figure 3. PTPRT promoter is hypermethylated in the colon and breast cancers of two mutation carriers.
(A+B) PTPRT promoter methylation obtained by MSP of two different promoter sites previously described. Panel A shows the methylation status of the CRC vs healthy colon tissue of member II:6 and a healthy colon pool. Panel B shows the methylation status of the breast cancer vs healthy breast of member II:4 and a commercial healthy breast sample. (C) PTPRT promoter methylation status (%) obtained by pyrosequencing for the same samples analyzed in A, together with methylated and unmethylated commercial controls.