Red marks on the OTOA locus represent every predicted difference between OTOA (NG_012973.2) and OTOAP1 (NC_000016.10, coordinates 22545698-22576865) when aligning genomic sequences. Black marks indicate positions where the OTOA nucleotide was found in the nanopore sequencing mapping, whereas red marks indicate positions where the OTOAP1 nucleotide was found. This analysis indicates that the conversion was at least 9kbp in proband 1 but less than 1kbp and restricted to exon 22 in proband 2.