4.8 Oxidative burst- ROS measurement
ROS measurement as previously described (Leibman-Markus et al., 2017).
Leaf disks of 0.5 cm in diameter were harvested from leaves 4-5 of 5-6
week old M82 Mock and root-zone warmed tomato plants. Disks were
floated in a white 96-well plate (SPL Life Sciences, Korea) containing
250 μl distilled water for 4–6 h at room temperature. After washing,
water was removed and a ROS measurement reaction containing 1 mM flg-22
or water was added. Light emission was measured for 30 minutes using a
luminometer (Tecan Spark, Switzerland). Each experiment was repeated
four times with 13 technical replicates (Ntotal=52).