4.9 Data analysis
All experimental data is presented as average ±SEM. Differences between
two groups were analyzed for statistical significance using a two-tailed
t-test. Differences among three groups or more were analyzed for
statistical significance with a one-way ANOVA. Regular ANOVA was used
for groups with equal variances, and Welch’s ANOVA for groups with
unequal variances. When a significant result for a group in an ANOVA was
returned, significance in differences between the means of different
samples in the group were assessed using a post-hoc test. The Tukey test
was employed for samples with equal variances when the mean of each
sample was compared to the mean of every other sample. The Bonferroni
test was employed for samples with equal variances when the mean of each
sample was compared to the mean of a control sample. The Dunnett test
was employed for samples with unequal variances. All statistical
analyses were conducted using Prism8TM.