Inhibition of Ca2+ flux is protective against PV-IgG-induced pathogenic effects in human skin ex vivo
In human skin samples PV-IgG induced blistering (Figure 4a) where typical tombstoning was observed (Figure 4/S4). At the blistering sites, PV-IgG was enriched and in non-affected regions it was deposited at cell borders (Figure S4a). DSG 1 and 3 staining was fragmented and redistributed to the cytosol in response to incubation with autoantibodies (Figure 4c/S4b). Moreover, actin staining was almost abolished at blistering sites (Figure 4d). All pathogenic effects were completely blocked by inhibition of PLC (Figure 4/S4). This demonstrates that PLC is important for PV-IgG-induced blistering of human skin.