Inhibition of Ca2+ flux is protective
against PV-IgG-induced pathogenic effects in human skin ex vivo
In human skin samples PV-IgG induced blistering (Figure 4a) where
typical tombstoning was observed (Figure 4/S4). At the blistering sites,
PV-IgG was enriched and in non-affected regions it was deposited at cell
borders (Figure S4a). DSG 1 and 3 staining was fragmented and
redistributed to the cytosol in response to incubation with
autoantibodies (Figure 4c/S4b). Moreover, actin staining was almost
abolished at blistering sites (Figure 4d). All pathogenic effects were
completely blocked by inhibition of PLC (Figure 4/S4). This
demonstrates that PLC is important for PV-IgG-induced blistering
of human skin.