2K1C Renal Stenosis Model:
Eight week old C57B6/J male mice (Jackson Laboratories) were
anesthetized with 2-3% isoflurane in 100% O2. Through
a retroperitoneal incision, the right renal artery was carefully
isolated from the renal nerve. A 0.5 mm polytetrafluoroethylene catheter
(ID: 0.008 X OD: 0.014; Braintree Scientific, SUBL140) was cut
longitudinally, placed around the right renal artery, distal to the
adrenal artery, and secured with two 10-0 sutures. Right renal arteries
were exposed with no clip placed for sham control procedures. Animals
were treated by subcutaneous injection of 0.03 mg/kg buprenorphine
twice/day for 48 hours (Henry Schein Inc.) and 2 mg/kg enrofloxacin
(Norbrook Laboratories) as previously published (Ong, et al., 2019).
Mice were fed a 0.1% NaCl diet as previously described (DeLalio, et
al., 2020), and animals were sacrificed 19-21 days following renal clip
or sham surgery.