2K1C Renal Stenosis Model:
Eight week old C57B6/J male mice (Jackson Laboratories) were anesthetized with 2-3% isoflurane in 100% O2. Through a retroperitoneal incision, the right renal artery was carefully isolated from the renal nerve. A 0.5 mm polytetrafluoroethylene catheter (ID: 0.008 X OD: 0.014; Braintree Scientific, SUBL140) was cut longitudinally, placed around the right renal artery, distal to the adrenal artery, and secured with two 10-0 sutures. Right renal arteries were exposed with no clip placed for sham control procedures. Animals were treated by subcutaneous injection of 0.03 mg/kg buprenorphine twice/day for 48 hours (Henry Schein Inc.) and 2 mg/kg enrofloxacin (Norbrook Laboratories) as previously published (Ong, et al., 2019). Mice were fed a 0.1% NaCl diet as previously described (DeLalio, et al., 2020), and animals were sacrificed 19-21 days following renal clip or sham surgery.