Statistics:
Statistical analyses were performed using Graphpad Prism Software 7.0d.
For wire myography, p-values represent significance by two-way ANOVA
analysis with Sidak multiple comparisons tests for comparisons between
groups at each vasodilator concentration. Based upon normality using a
Shapiro-Wilk test, p-values for statistics in qPCR, Western blot, and
immunostaining were assessed using either an unpaired two-tailedt -test or unpaired two-tailed t -test with Welch’s
correction. Symbols were consistent throughout wherein * denotes
p<0.05, ** denotes p<0.01, *** denotes
p<0.001, and **** p<0.0001.