3.2 Altered T-cell subset frequencies in lymphoid tissue

CD3+ T cells were subdivided into αβ T cells (CD3+/ γδ TCR) and γδ T cells (CD3+/ γδ TCR). αβ T cells in domestic pigs showed decreases 5 dpi in blood, spleen, lung, and liver with corresponding increased γδ T-cell frequencies (Figure 1A, B). The T-cell frequencies in domestic pigs returned to control levels 10 dpi, except for αβ T-cell increases in the liver (Figure 1A). In wild boar, we detected temporarily increased αβ T-cell frequencies in blood and spleen 4 dpi but no other changes to baseline level (Figure 1A) and largely unaffected γδ T-cell frequencies over the study period, except for corresponding decreases in γδ T-cell frequencies in blood and spleen (Figure 1B). No changes were detected in the gastro-hepatic lymph nodes (ghLN, Lymphonodi hepatici or gastrici ) of both subspecies.