Marker Clone Isotype Conjugate Source Dilution
CD2 MSA4 mouse IgG2a in-house 1:100
CD3ε PPT3 mouse IgG1 APC Southern Biotech 1:500
CD3ε PPT3 mouse IgG1 PE Southern Biotech 1:500
CD4a 74-12-4 mouse IgG2b PerCp-Cy5.5 BD 1 :100
CD8a 76-2-11 mouse IgG2a FITC Southern Biotech 1:100
CD8b PG164A mouse IgG2a in-house 1:1000
Foxp3 FJK-16s mouse IgG1 PE-Cy7 ebioscience 1:200
gdTCR PPT16 mouse IgG2b in-house 1:100
Ki-67 B56 mouse IgG1 BV421 BD Biosciences 1:40
Perforin dG9 mouse IgG2b Alexa 647 Biolegend 1:20
T-bet 4B10 mouse IgG1 APC Biolegend 1:500
IgG2a polyclonal Goat IgG APC-Cy7 Southern Biotech 1:250
IgG2b polyclonal Goat IgG PE Southern Biotech 1:400
IgG2b polyclonal Goat IgG PE-Cy7 Southern Biotech 1:400