Apocynin prevents the suppression of myogenic factor expression
and myofiber wasting induced by CSE and
H2O2
Under unstimulated conditions, apocynin treatment had no effect on
myotube diameters (Figure 5A and F). However, treatment with apocynin
prevented myofiber wasting (i.e. reduction in myofiber size) elicited by
different concentrations of H2O2 (Figure
5A) or CSE (Figure 5F). Apocynin treatment completely attenuated the
up-regulated expression of Nox2 (Figure 5B and G) and Il-6(Figure 5C and H) driven by H2O2 or CSE.
Moreover, apocynin normalized the expression of Igf1-ea and
Igf1-eb , suggesting apocynin was able to antagonize the
oxidant-dependent and -independent effects of CSE on myofiber wasting.