Immediately following the contraction protocol, the mouse was removed from the apparatus and euthanized with an overdose of anaesthetic (Sodium pentobarbitone; 240mg/kg: Virbac Pty. Ltd., Australia) via an i.p. injection to continue with tissue collection and the procedures described below. Muscle mass was measured using an analytical balance after the tendons and other non-muscle tissues have been removed and after brief contact with absorbent material such as filter paper to remove excess solution.