Sanger sequencing-
In our sequenced samples we identified 15 unique bacterial genera. The majority of isolates were in the genus Bacillus(~13% of our sequenced isolates) but a sizable amount belonged to other taxonomic groups including Microvirga(~6 %), Micromonospora (~5 %),Skermanella (~5 %), and Paenibacillus(~3 %). Isolates from four genera were isolated from our 1950 sample. We identified isolates from 12 genera in our two nodules taken from the 2004 specimen. We found isolates from three genera from our nodule taken from a 2015 specimen (Fig. 3).