Orthotopic lung cancer model in mice
Mice were anesthetized with chloral hydrate (300 mg/kg) via
intraperitoneal injection and placed in the right lateral decubitus
position.
A
total of 1 × 106 cells in 50 µL RPMI-1640 medium and
50 µL Matrigel were injected into the left lung
parenchyma
through the left rib cage. Mice were monitored daily. Mice were
euthanized by pentobarbital sodium, and the lung tissues were removed.