2.4 PK Analyses
Emodepside concentrations were determined by a validated high performance liquid chromatography-tandem mass spectrometry assay. Deuterated emodepside-D16 was used as the internal standard. The lower limit of quantitation for emodepside in plasma was 1 ng/mL. Values below this limit were not used to calculate the PK parameters, except values that were below the limit of quantification before Cmax, which were set to zero.
The highest observed plasma concentration (Cmax) was determined directly from concentration-time data, as was the time to reach maximum plasma concentration (tmax). The area under the plasma concentration-time curve from time zero (pre-dose) to the time of last quantifiable concentration (AUC0‐last) was calculated using the linear‐log trapezoidal rule. Elimination half‐life (t1/2) was calculated by the equation 1n2/λz.