2.4 PK Analyses
Emodepside concentrations were determined by a validated high
performance liquid chromatography-tandem mass spectrometry assay.
Deuterated emodepside-D16 was used as the internal
standard. The lower limit of quantitation for emodepside in plasma was 1
ng/mL. Values below this limit were not used to calculate the PK
parameters, except values that were below the limit of quantification
before Cmax, which were set to zero.
The highest observed plasma concentration (Cmax) was
determined directly from concentration-time data, as was the time to
reach maximum plasma concentration (tmax). The area
under the plasma concentration-time curve from time zero (pre-dose) to
the time of last quantifiable concentration (AUC0‐last)
was calculated using the linear‐log trapezoidal rule. Elimination
half‐life (t1/2) was calculated by the equation
1n2/λz.