2.8 | Overexpression of PPARγ2 in SMCs
The PPARγ2 plasmids (ORIGENE, Cat# MR208132) or its control CMV-GFP
plasmids (ORIGENE, Cat# PS100040) were transfected into SKI SMCs
according to Effectene® Transfection Reagent Handbook (Qiagen,
Cat#301305). The fluorescence intensity of GFP was observed by
fluorescence microscope (Leica, DMi8) to evaluate the transfection
efficiency. SMCs were collected 48 hr later for protein analysis and
cell function studies.