2.8 | Overexpression of PPARγ2 in SMCs
The PPARγ2 plasmids (ORIGENE, Cat# MR208132) or its control CMV-GFP plasmids (ORIGENE, Cat# PS100040) were transfected into SKI SMCs according to Effectene® Transfection Reagent Handbook (Qiagen, Cat#301305). The fluorescence intensity of GFP was observed by fluorescence microscope (Leica, DMi8) to evaluate the transfection efficiency. SMCs were collected 48 hr later for protein analysis and cell function studies.