Parentheses represent the number of independent experiments, each with duplicate wells. Kinetic parameters were derived from a global association fit from the full 90 minute time course (VEGF₁₆₅b-TMR) or the initial 20 minutes (VEGF₁₆₅a-TMR). Experiments with LgBiT-VEGFR2 and HiBiT-NRP1 (HiBiT Complex) are shown in Figure 7a and c. Kinetic experiments with LgBiT-VEGFR2 and SmBiT-NRP1 (SmBiT Complex) are shown in Figure 8a and c.