Figure 2. IMC mapping identifies predominance of
CD8+ T cell clusters in COVID-MDR.
A, UMAP representation of all single cells depicting the
different identified cell-types (upper graph) and all cells colored by
indication (lower graph). B, Boxplots for the fractions of each
cell-type per image split and colored by indication. (COVID-MDR, MDR,
DRESS, HC). C, Heatmap of z-scored average expression for each
marker and cell-type. D, Example images of classified
cell-types in COVID-MDR, DRESS and MDR. The scale bar (white line) is
200 µm in all images. E, Example images of the expression of
CD3 (green), CD4 (magenta), CD8 (red), GrzB (yellow) and DNA (blue). The
large images depict the overlayed colors, and the white box marks the
zoomed in areas on the right side depicting the individual markers. The
scale bar (white line) is 100 µm in all images.