Association to drivers
Within-population genetic diversity (nucleotide diversity π and
heterozygosity He) was not influenced by lake area (π:
Spearman’s rho = 0.03, p = 0.95, He: rho = -0.06,p = 0.88), connection (π: rho = 0.43, p = 0.25,
He: rho = 0.53, p = 0.15), salinity (π: rho =
0.37, p = 0.33, He: rho = 0.24, p = 0.53)
(Supplemental Table 6, Supplemental Figure 7). However, there appeared
to be a trend towards higher nucleotide diversity with lower water
temperatures (rho = -0.61, p = 0.08), but not for heterozygosity
(rho = -0.20, p = 0.60).
Mantel tests indicated no correlation between the geographic and genetic
distance matrices over all filter options (Figure 4A, Supplemental Table
7, r = 0.007, p = 0.504). Finding no correlation refutes the
isolation-by-distance hypothesis and indicates other factors might
explain the distribution of S. diversicolor genetic diversity.
However, the genetic distance matrix also did not correlate with
matrices of environmental distance (r = 0.002, p = 0.503, Figure
4B) or connection distance (r = 0.041, p = 0.441, Figure 4C).