Cell culture and treatment
SH-SY5Y cells (ATCC, Cat# CRL-2266, RRID:CVCL_0019) were cultured at 37 in a humidified atmosphere of 5% CO2 and 95% air with DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin and streptomycin. The cells were incubated for 1 hour prior to MPP+ treatment with Sinapic acid (SA), SR8278 (SR, REV-ERB α antagonist) and GSK4112 (GSK, REV-ERB α agonist) depending on the experimental design. The stock solutions of each reagent were diluted to the final concentrations in medium prior to use.