ATP concentration
The concentration of ATP was detected by a commercial ATP assay kit (Cat# K354, Biovision). After deproteinization of lysates using a Deproteinization Sample Preparation Kit (Cat# K808, Biovision) to rapidly block ATP consumption, deproteinized lysates and reagents mixed and put in the 96-well plate. The plate was incubated for 30 min at room temperature and then the absorbance was measured at 570 nm.