Cell viability assay
Cell viability was detected by using MTT assay kit (EZ-Cytox kit, Cat#
EZ-3000, DAEILLAM Co, Ltd, Seoul, Republic of Korea) according to the
instruction of manufacturer. Briefly, SH-SY5Y cells were seeded at a
density of 6 \(\times\) 104cells·well-1 in 96-well culture plate and incubated
with each reagent and EZ-Cytox solution for 1 h at 37. The
absorbance of culture plate was measured at 450 nm with a microplate
reader (TECAN, Switzerland).