Cell culture and treatment
SH-SY5Y cells (ATCC, Cat# CRL-2266, RRID:CVCL_0019) were cultured at
37 in a humidified atmosphere of 5% CO2 and 95%
air with DMEM supplemented with 10% heat-inactivated FBS and 1%
penicillin and streptomycin. The cells were incubated for 1 hour prior
to MPP+ treatment with Sinapic acid (SA), SR8278 (SR,
REV-ERB α antagonist) and GSK4112 (GSK, REV-ERB α agonist) depending on
the experimental design. The stock solutions of each reagent were
diluted to the final concentrations in medium prior to use.