Data and statistical analysis
The data and statistical analysis complied with the recommendations on experimental design and analysis in pharmacology made by theBritish Journal of Pharmacology (Curtis et al., 2018). Sample sizes subjected to statistical analysis at least 5 animals per group (n=6), where n= number of independent values. Data were analyzed with Prism 7.0 software (GraphPad Software, Inc., San Diego, CA, USA, RRID:SCR_002798) using one-way ANOVA followed by the Bonferroni test for multiple comparisons. The results are expressed as the means ± S.E.M.