Animals
Male C57BL/6 mice (weight 25-28 g, 7 weeks old, Orient Bio Inc,
Seongnam, Korea, MGI Cat# 5656552, RRID:MGI:5656552) were kept a full
automatically temperature and humidity controlled room (22\(\pm\)3,
50%, 12 h light and dark cycle) with free access to food and water.
Animal experiments were followed by the Korea Institute of Science and
Technology Animal Care Committee guidelines and approved by the Korea
Institute of Science and Technology Animal Care and Use Committee
(KIST-2018-077). Furthermore, animal studies are reported in compliance
with the ARRIVE guidelines (McGrath & Lilley, 2015).
The mice were randomly divided into 5 groups in each animal experiments.
The group for the first study was composed like a vehicle-treated
control group (1, Control), SA 20 mg·kg-1-treated
control group (2, SA20), vehicle-treated MPTP group (3), SA 10
mg·kg-1-treated MPTP group (4, SA10), and SA 20
mg·kg-1-treated MPTP group (5, SA20). In the second
study, the 5 groups are vehicle-treated control group (1, Control),
vehicle-treated MPTP group (2), Ropinirole 5
mg·kg-1-treated MPTP group (3, RO), SA 20
mg·kg-1-treated MPTP group (4, SA), and both SA 20
mg·kg-1 and SR 25 mg·kg-1-treated
MPTP group (5, SA+SR). Mice were injected 30 mg·kg-1MPTP (i.p.) dissolved in PBS on five consecutive days with or without SA
(p.o), SR and ropinirole (i.p.), which were treated 30 min before MPTP
injection. SR was intraperitoneal administered at 30 min before SA
treatment in the combination of SA and SR group. The treatment dose of
SA was followed by previous neurological studies and all reagents were
dissolved in PBS, but SR was dissolved in 5:5:90
DMSO·cremophor-1·PBS-1 vehicle
followed by previous study of Welch et al. (Kim et al., 2010; Lee et
al., 2012; Welch, Billon, Valfort, Burris & Flaveny, 2017). After the
behavioral tests, the mice were euthanized with CO2inhalation and then SNpc tissues were collected and stored at -80.