Whole exome sequencing
151 genomic DNA was exacted from peripheral blood by Axy Prep Blood
Genomic DNA Mini prep Kit (Item No. 05119KC3). To fulfill the
requirements for genotyping, the concentration and integrality of the
all the exacted DNA samples were determined by Nanodrop-1000
spectrophotometer (Thermo Fisher, USA) and gel electrophoresis,
respectively. A total of 151 ICP patients samples were subjected to
carry out exome capture with BGI Exon Kit according to manufacturer’s
protocols. DNA library were constructed using combinatorial Probe Anchor
Ligation (cPAL) method. Each resulting qualified captured library was
then loaded on BGISEQ-500 sequencing platforms.