Supporting information
Additional supporting information may be found online in the Supporting Information section at the end of the article.
Table S1. Primes used for confirmed the variants in ABC transporters’ genes.
Table S2. Descriptive statistics of 26 clinical data of ICP individuals associated with these mutations.
Figure S1. The distribution and numbers of genetic variants from WES data for ICP. (A) The percentage of the types of genetic variants in ABC series genes. (B) The total number of genetic variants before and after quality control, and the novel variants. TSNPs: Total SNPs before quality control; QCSNPs: Total SNPs after quality control; NSNPs: Novel SNPs.
Figure S2. Sanger sequencing to validate the novel variants in ABC series genes. (A) ABCA genes; (B) ABCB genes; (C) ABCC genes; (D) ABCD-ABCG genes.
Figure S3. The evolutionary conservative analysis of ABC novel representative mutations. There mutations were highly conserved in vertebrate species.
Figure S4. The comparison of the expression level ofABCC6 , ABCE1 and ABCG5 genes between two healthy and 4 ICP patients in placental tissue.
Figure S5. Correlation coefficients among twenty-six clinical features. The dots indicate the significant (P < 0.05) correlation coefficients between each pair of features. Its size and colors separately represent the degree and direction of correlation coefficients.