Supporting information
Additional supporting information may be found online in the Supporting
Information section at the end of the article.
Table S1. Primes used for confirmed the variants in ABC
transporters’ genes.
Table S2. Descriptive statistics of 26 clinical data of ICP
individuals associated with these mutations.
Figure S1. The distribution and numbers of genetic variants
from WES data for ICP. (A) The percentage of the types of genetic
variants in ABC series genes. (B) The total number of genetic variants
before and after quality control, and the novel variants. TSNPs: Total
SNPs before quality control; QCSNPs: Total SNPs after quality control;
NSNPs: Novel SNPs.
Figure S2. Sanger sequencing to validate the novel variants in
ABC series genes. (A) ABCA genes; (B) ABCB genes; (C) ABCC genes; (D)
ABCD-ABCG genes.
Figure S3. The evolutionary conservative analysis of ABC novel
representative mutations. There mutations were highly conserved in
vertebrate species.
Figure S4. The comparison of the expression level ofABCC6 , ABCE1 and ABCG5 genes between two healthy
and 4 ICP patients in placental tissue.
Figure S5. Correlation coefficients among twenty-six clinical
features. The dots indicate the significant (P < 0.05)
correlation coefficients between each pair of features. Its size and
colors separately represent the degree and direction of correlation
coefficients.