Figure legends
Figure 1. 2-MAGs percentage based on maximum theoretical yield at different reaction times using TLL adsorbed on Sepabeads C-18 and TLL commercial at studied reaction times and temperatures.
Figure 2. Reaction kinetics of 2-MAGs production from echium oil at optimum temperature (25 °C) catalyzed by TLL commercial (a), TLL adsorbed on Sepabeads C-18 (b) and TLL adsorbed on Sepabeads C-18 using equivalent enzyme loading to commercial biocatalyst (c). (●) TAGs, (▲) DAGs, (■) 2‑MAGs.
Figure 3. Fractionation of synthesized 2-MAG by SPE at optimal reaction time analyzed by HPLC‑ELSD: standard mixture of different species of lipids (a), original reaction (b), SPE fraction composed by FAEEs (c) and SPE fraction composed by pure 2-MAGs (d).
Figure 4. Recovered activity (%) of TLL adsorbed on Sepabeads C-18 and TLL commercial for 2‑MAGs production after five reaction cycles. Reutilization was evaluated in reaction cycles of 2 h (optimal reaction time).
Figure 5. Reaction kinetic of transesterification reaction between synthesized 2-MAGs and caprylic acid ethyl esters catalyzed by RML commercial (a) and RML adsorbed on Sepabeads C‑18 (b). (●) STAGs, (▲) DAGs, (■) 2‑MAGs.
Figure 6. Chromatograms obtained by HPLC-ELSD of transesterification reaction between synthesized 2-MAGs and caprylic acid ethyl esters: reaction products at initial reaction time (a), reaction products after 20 min (b) and reaction products after 1 h (c).