Soil fungal sampling
Soil samples were collected from each experimental plot from August
25-28, 2020 using a 2.2 cm diameter soil sampling probe. The surface
plant litter layer was removed, and four soil cores per plot were
extracted to a depth of 10 cm. The four cores were composited and
homogenized using a 2 mm sieve and roots were removed. Soil probes and
sieves were sterilized with bleach between plots to reduce the chance of
cross-contamination. Soils were then frozen at −20°C until DNA
extraction.