Flow cytometry analysis
After PEDV infection, RBCs were pre-labeled with CM-Dil Dye and stained with a monoclonal antibody against PEDV-N protein. PBMCs were stained with an antibody specific for porcine CD3 (BD Biosciences). To detect PEDV, cells were resuspended in fixation/permeabilization solution (BD Cytofix/Cytoperm kit, BD Pharmingen) and stained with antibody against PEDV-N protein. BD FACS Calibur were used for flow cytometry and FlowJo X.10.0.7 was used to analyze the data.