Immunohistochemistry (IHC) and Immunofluorescence assay (IFA)
All piglets were euthanized and sacrificed to collect tissues. After fixation, tissues were dehydrated, embedded and sectioned. Subsequently, tissues were stained with primary antibody against PEDV-N protein for observing the distribution of PEDV by IHC staining. For IFA, after staining with antibody against PEDV-N protein, tissues were incubated with secondary antibodies Alex-Flour 594 goat anti-Mouse IgG (Invitrogen), and the nuclei was counterstained with DAPI (Beyotime) for 5 min.