Figure legends
Figure 1. The provocative concentration causing a 20% reduction in the forced expiratory volume in 1 second (PC20) at 1 and 3 months after discharge. ○: children with previous asthma diagnosis; △: children without previous asthma diagnosis.
Figure 2. Resistance of respiratory system (Rrs) to methacholine challenge in asthma model mice infected with vehicle (mock), A(H1N1)pdm09, or seasonal H1N1 (A/Puerto Rico) at 3 (A), 7 (B), and 10 (C) days post-infection. Data are presented as means ± standard error of the mean (SEM); ●: asthma – mock; ■: asthma - A(H1N1)pdm09; ▲: asthma – seasonal H1N1; □: non-asthma – A(H1N1)pmd09. Differences between means are presented as follows: asthma - mock vs. asthma - seasonal H1N1, * p < 0.05; asthma - seasonal H1N1 vs. asthma - A(H1N1)pdm09, † p < 0.05; asthma - A(H1N1)pdm09 vs. asthma – mock, ‡ p < 0.05; and asthma – A(H1N1)pdm09 vs. non-asthma – A(H1N1)pdm09, § p < 0.05.
Figure 3. Tissue damping (G) of methacholine challenge in asthma model mice infected with vehicle (mock), A(H1N1)pdm09, or seasonal H1N1 (A/Puerto Rico) at 3 (A), 7 (B), and 10 (C) days post-infection. Data are presented as means ± standard error of the mean (SEM); ●: asthma – mock; ■: asthma - A(H1N1)pdm09; ▲: asthma – seasonal H1N1; □: non-asthma – A(H1N1)pmd09. Differences between means are presented as follows: asthma - mock vs. asthma - seasonal H1N1, * p < 0.05; asthma - seasonal H1N1 vs. asthma - A(H1N1)pdm09, † p < 0.05; asthma - A(H1N1)pdm09 vs. asthma - mock, ‡ p < 0.05; and asthma – A(H1N1)pdm09 vs. non-asthma – A(H1N1)pdm09, § p < 0.05.
Figure 4. Changes in airway hyperresponsiveness of A(H1N1)pdm09-infected mice at 3, 7, and 10 days post-infection. (A) Resistance of respiratory system (Rrs) to methacholine challenge. (B) Tissue damping (G) of methacholine challenge. Data are presented as means ± standard error of the mean (SEM); ●: day 3, ■: day 7, ▲: day 10. Differences between means are presented as follows: day 3vs. day 7, * p < 0.05; day 3 vs. day 10, † p < 0.05; and day 7 vs. day 10, ‡ p < 0.05.
Figure 5. Histopathological findings after influenza infection. Photomicrographs of haematoxylin and eosin-stained lung tissue at 3 (A), 7 (B), and 10 (C) days post-infection with mock, seasonal H1N1 (A/Puerto Rico), or A(H1N1)pdm09 influenza virus. Representative data are shown for tissues from one of four to six independent mice per group.