Sensitization and allergen challenge in a mouse model of bronchial asthma
BALB/c mice aged 6–8 weeks were obtained from Chiyoda Kaihatsu Co. (Tokyo, Japan) and sensitized and challenged with grade II ovalbumin (Sigma–Aldrich., St. Louis, MO, USA) as previously described12-14. All animal experimentation procedures were approved by the Institutional Animal Care and Use Committee of Yamaguchi University (No. 29-S01), and all methods were conducted in accordance with approved guidelines. Additionally, animal experiments conformed to the revised Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council “Guide for the Care and Use of Laboratory Animals” published by the National Academy Press, Washington, D.C. 1996.