Sensitization and allergen challenge in a mouse model of
bronchial asthma
BALB/c mice aged 6–8 weeks were obtained from Chiyoda Kaihatsu Co.
(Tokyo, Japan) and sensitized and challenged with grade II ovalbumin
(Sigma–Aldrich., St. Louis, MO, USA) as previously
described12-14. All animal experimentation procedures
were approved by the Institutional Animal Care and Use Committee of
Yamaguchi University (No. 29-S01), and all methods were conducted in
accordance with approved guidelines. Additionally, animal experiments
conformed to the revised Institute of Laboratory Animal Resources,
Commission on Life Sciences, National Research Council “Guide for the
Care and Use of Laboratory Animals” published by the National Academy
Press, Washington, D.C. 1996.