Figure legends
Figure 1. Resistance of respiratory system (Rrs) to
methacholine challenge in asthma model mice infected with vehicle
(mock), A(H1N1)pdm09, or seasonal H1N1 (A/Puerto Rico) at 3 (A), 7 (B),
and 10 (C) days post-infection. Data are presented as means ± standard
error of the mean (SEM); ○: asthma – mock; □: asthma - A(H1N1)pdm09; △:
asthma – seasonal H1N1; ◆: non-asthma – A(H1N1)pmd09. Differences
between means are presented as follows: asthma - mock vs. asthma -
seasonal H1N1, * p < 0.05; asthma - seasonal H1N1 vs. asthma -
A(H1N1)pdm09, † p < 0.05; asthma - A(H1N1)pdm09 vs. asthma –
mock, ‡ p < 0.05; and asthma – A(H1N1)pdm09 vs. non-asthma –
A(H1N1)pdm09, § p < 0.05.
Figure 2. Tissue damping (G) of methacholine challenge in
asthma model mice infected with vehicle (mock), A(H1N1)pdm09, or
seasonal H1N1 (A/Puerto Rico) at 3 (A), 7 (B), and 10 (C) days
post-infection. Data are presented as means ± standard error of the mean
(SEM); ○: asthma – mock; □: asthma - A(H1N1)pdm09; △: asthma –
seasonal H1N1; ◆: non-asthma – A(H1N1)pmd09. Differences between means
are presented as follows: asthma - mock vs. asthma - seasonal H1N1, * p
< 0.05; asthma - seasonal H1N1 vs. asthma - A(H1N1)pdm09, † p
< 0.05; asthma - A(H1N1)pdm09 vs. asthma - mock, ‡ p
< 0.05; and asthma – A(H1N1)pdm09 vs. non-asthma –
A(H1N1)pdm09, § p < 0.05.
Figure 3. Changes in airway hyperresponsiveness of
A(H1N1)pdm09-infected mice at 3, 7, and 10 days post-infection. (A)
Resistance of respiratory system (Rrs) to methacholine
challenge. (B) Tissue damping (G) of methacholine challenge. Data are
presented as means ± standard error of the mean (SEM); ○: day 3, □: day
7, △: day 10. Differences between means are presented as follows: day 3vs. day 7, * p < 0.05; day 3 vs. day 10, † p
< 0.05; and day 7 vs. day 10, ‡ p < 0.05.
Figure 4. Histopathological findings after influenza infection.
Photomicrographs of haematoxylin and eosin-stained lung tissue at 3 (A),
7 (B), and 10 (C) days post-infection with mock, seasonal H1N1 (A/Puerto
Rico), or A(H1N1)pdm09 influenza virus. Representative data are shown
for tissues from one of four to six independent mice per group.
Figure 5. The provocative concentration causing a 20%
reduction in the forced expiratory volume in 1 second
(PC20) at 1 and 3 months after discharge.