Figure 1 – Initial
screen of small molecule culture additives identifies nocodazole, M344
and z-VAD-fmk as novel putative enhancers of rAAV genome titre. Cells
were analysed 72 hours post transfection (HPT) for rAAV8 genome titre,
shown here as the fold change relative to rAAV8 producing cells
containing no small molecule enhancers (Ctrl). Nocodazole, M344, and
z-VAD-fmk are highlighted (dashed boxes) (A ). Mean cell volume
vs genome titre fold change is shown in (B ), dashed lines
indicate control values. R2 and p-value calculated
from Pearson correlation coefficient of mean cell volume vs genome
titre. Data shown in A are mean ± SEM for two independent
experiments carried out in technical duplicate, with the exception of
TUDCA, TMAO, Betaine and LiCl which are mean only data from one
independent experiment carried out in technical duplicate (data from
these experiments is omitted in B ). NaBu - Sodium butyrate; VPA
– Valproic acid; TUDCA - Tauroursodeoxycholic acid; TMAO -
Trimethylamine N-oxide; LiCl – Lithium chloride