Figure 2 – Addition of
nocodazole to rAAV8 producing cells to increase genome titre is
time-dependent. rAAV8 producing cells were cultured in 24-well
microplates and 4 µM nocodazole was added at 0-, 4-, 24-, or 48-HPT or
left untreated (Ctrl). At 72 HPT cells were harvested and viable cell
density (VCD) (A ), viability (B ) and mean cell volume
(C ) was measured. Final crude genome titre expressed as a fold
change relative to Ctrl is shown in (D ). Data shown for VCD,
viability and mean cell diameter are the mean ± S.E.M. n >
3 independent biological replicates. Data shown for genome titre fold
change are the mean ± S.E.M. n ≥ 3 independent biological replicates.
Data were analysed by one-way ANOVA followed by post-hoc Holm-Šídák
multiple comparisons test with respect to Ctrl. * p < 0.05, **
p < 0.01, *** p < 0.001, **** p < 0.0001.