Figure 1 – Initial screen of small molecule culture additives identifies nocodazole, M344 and z-VAD-fmk as novel putative enhancers of rAAV genome titre. Cells were analysed 72 hours post transfection (HPT) for rAAV8 genome titre, shown here as the fold change relative to rAAV8 producing cells containing no small molecule enhancers (Ctrl). Nocodazole, M344, and z-VAD-fmk are highlighted (dashed boxes) (A ). Mean cell volume vs genome titre fold change is shown in (B ), dashed lines indicate control values. R2 and p-value calculated from Pearson correlation coefficient of mean cell volume vs genome titre. Data shown in A are mean ± SEM for two independent experiments carried out in technical duplicate, with the exception of TUDCA, TMAO, Betaine and LiCl which are mean only data from one independent experiment carried out in technical duplicate (data from these experiments is omitted in B ). NaBu - Sodium butyrate; VPA – Valproic acid; TUDCA - Tauroursodeoxycholic acid; TMAO - Trimethylamine N-oxide; LiCl – Lithium chloride