Figure 3 – Increased genome titre in nocodazole treated cells is maintained in larger scale cultures and across two separate serotypes. 30 mL shake flask cultures were transfected with rAAV8 or rAAV9 producing plasmids and cultured for 72 HPT in the presence (rAAV8: ; rAAV9: ) or absence (rAAV8: ; rAAV9: ■) of 4 µM nocodazole added 4 hours post-transfection. Cultures were measured daily for viable cell density (VCD) (A – solid lines), viability (A – dashed lines) and mean cell volume (B ). Fold change analysis of genome titre for both serotypes in the presence (+) or absence (–) of 4 µM nocodazole at 72 HPT is shown in (C ). Data shown are the mean ± S.E.M. n = 3 independent biological replicates. Fold change between nocodazole treated and untreated cultures was analysed for each serotype separately by Student’s unpaired two-tailed t-test with respect to untreated cultures. ** p < 0.01, *** p < 0.001.