Figure 6 – Genome titre improvements are retained in larger scale cultures. 30mL shake flask cultures were transfected with rAAV8 producing plasmids with no chemical additives (■), with the addition of 4µM nocodazole at 4 hours post-transfection (), or with the addition of 4 µM nocodazole and 2.5 µM M344 at 4 hours post-transfection (). Cultures were measured daily for VCD (A – solid lines) and viability (A – dashed lines). Genome titre was measured 72 hours post-transfection and is shown as fold change with respect to untreated cultures (B ). Data shown are the mean ± S.E.M. n = 3 independent biological replicates. Data were analysed by one-way ANOVA followed by post-hoc Holm-Šídák multiple comparisons test with respect to untreated cells (-/-). * p < 0.05, ** p < 0.01