Figure 6 – Genome titre
improvements are retained in larger scale cultures. 30mL shake flask
cultures were transfected with rAAV8 producing plasmids with no chemical
additives (■), with the addition of 4µM nocodazole at 4 hours
post-transfection (), or with the addition of 4 µM nocodazole and 2.5 µM
M344 at 4 hours post-transfection (). Cultures were measured daily for
VCD (A – solid lines) and viability (A – dashed
lines). Genome titre was measured 72 hours post-transfection and is
shown as fold change with respect to untreated cultures (B ).
Data shown are the mean ± S.E.M. n = 3 independent biological
replicates. Data were analysed by one-way ANOVA followed by post-hoc
Holm-Šídák multiple comparisons test with respect to untreated cells
(-/-). * p < 0.05, ** p < 0.01