Figure 3 – Increased
genome titre in nocodazole treated cells is maintained in larger scale
cultures and across two separate serotypes. 30 mL shake flask cultures
were transfected with rAAV8 or rAAV9 producing plasmids and cultured for
72 HPT in the presence (rAAV8: ; rAAV9: ) or absence (rAAV8: ; rAAV9: ■)
of 4 µM nocodazole added 4 hours post-transfection. Cultures were
measured daily for viable cell density (VCD) (A – solid
lines), viability (A – dashed lines) and mean cell volume
(B ). Fold change analysis of genome titre for both serotypes in
the presence (+) or absence (–) of 4 µM nocodazole at 72 HPT is shown
in (C ). Data shown are the mean ± S.E.M. n = 3 independent
biological replicates. Fold change between nocodazole treated and
untreated cultures was analysed for each serotype separately by
Student’s unpaired two-tailed t-test with respect to untreated cultures.
** p < 0.01, *** p < 0.001.