Figure 2 – Addition of nocodazole to rAAV8 producing cells to increase genome titre is time-dependent. rAAV8 producing cells were cultured in 24-well microplates and 4 µM nocodazole was added at 0-, 4-, 24-, or 48-HPT or left untreated (Ctrl). At 72 HPT cells were harvested and viable cell density (VCD) (A ), viability (B ) and mean cell volume (C ) was measured. Final crude genome titre expressed as a fold change relative to Ctrl is shown in (D ). Data shown for VCD, viability and mean cell diameter are the mean ± S.E.M. n > 3 independent biological replicates. Data shown for genome titre fold change are the mean ± S.E.M. n ≥ 3 independent biological replicates. Data were analysed by one-way ANOVA followed by post-hoc Holm-Šídák multiple comparisons test with respect to Ctrl. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.