Fig S1 Distribution of 17-mer frequency in Portunus
trituberculatus genome. The volume of K-mer is plotted against the
frequency at which they occur. The left-hand peak at low frequency and
high volume represented K-mer containing essentially random sequencing
errors. The main volume peak of K-mer was 41. The genome size was
estimated as 1.2 Gb by the formula ’Genome
size=total_kmer_num/kmer_depth’
Fig S2 Comparison of gene features among P trituberculatus ,Tetranychus urticae , Drosophila melanogaster ,Daphnia pulex , Ixodes scapularis , Parasteatoda
tepidariorum , Penaeus vannamei , Tribolium castaneum .
Fig S3 Distribution of divergence rate of each type of TEs in theP.trituberculatus genome. The divergence rate was calculated
between the identified TE elements in the genome and the consensus
sequence in the de novo library we used.
Fig S4 The distribution of genes in different species
Fig S5 The statistics of KEGG pathway enrichment of expanded families
Fig S6 The statistics of KEGG pathway enrichment of identified specific
families
Fig S7 the expression of genes in ribosome pathway in different stages
of development, molting stage, low salt stress and pathogen infection
Fig S8 Trend of DEGs during salinity stress
Fig S9 KEGG enrichment of down-regulated expanded and specific DEGs
during salt stress
Fig S10 KEGG enrichment of up-regulated expanded and specific DEGs
during salt stress
Fig S11 KEGG enrichment analysis of growth-related genes
Fig S12 RT-PCR of Ptdmrt in male and female tissues of P.
trituberculatus
Table S1 Primers used in this study.xlsx
Table S2 General statistics of SNP in P.trituberculatus genome