Statistical analysis
All immunohistochemical markers were expressed as units in the tables
and graphs for clarity, but they represent the result of thedensity of cells per square millimeter enumerated in the
superficial and deep dermis of the samples obtained.
The nonparametric Mann–Whitney test was used to compare the markers,
for the entire study population with CSU and control skin, as well as
the relationship of the markers with gender and ASST. Spearman’s
correlation coefficients were used to measure the relationship between
immunohistochemical markers with clinical examinations, age, urticaria
course, and the 7-day urticaria activity score (UAS7). The level of
significance was 5% (Software: XLSTAT 2019).