Statistical analysis
All immunohistochemical markers were expressed as units in the tables and graphs for clarity, but they represent the result of thedensity of cells per square millimeter enumerated in the superficial and deep dermis of the samples obtained.
The nonparametric Mann–Whitney test was used to compare the markers, for the entire study population with CSU and control skin, as well as the relationship of the markers with gender and ASST. Spearman’s correlation coefficients were used to measure the relationship between immunohistochemical markers with clinical examinations, age, urticaria course, and the 7-day urticaria activity score (UAS7). The level of significance was 5% (Software: XLSTAT 2019).