Coagulation variables and activity and plasma nitric oxide levels
The plasma fibrinogen levels in RBC/PPP group gradually decreased but was maintained over 110 mg/dL even at the end of the experiment because PPP contained a certain amount of coagulation factors, including fibrinogen (Figure 4A). In contrast, the plasma fibrinogen levels continuously decreased and reached 20 mg/dL in both HbV and HES groups (Figure 7A). The values of AT III in both HbV and HES groups were significantly shorter than those in RBC/PPP group at 45 (p <0.05) and 60 min (p <0.01) (Figure 4B). Similarly, the PT was significantly lower in the HbV and HES groups than in the RBC/PPP group at the end of the experiment (p <0.01) (Figure 4C). Regarding APTT, the value beyond 30 min was set as reference because the value in over 75 sec could not be measured in this analysis (Figure 4D).
Regarding coagulation activity, ACT was significantly prolonged in both the HbV and HES groups at the end of experiment compared with that in the RBC/PPP group (p <0.05) (Figure S3A). As for clot rate, all three groups showed similar decreases in the clot rate with no significant differences (Figure S3B). There were also no significant differences in plasma NOx levels among the three groups throughout the experiment, and HbV group showed no significant reduction of NOx levels (Figure S3C).