Analyses of plasma nitric oxide
Plasma NO2-/NO3-(nitric oxide, NOx) levels were measured every 15 min. Blood samples were collected in heparin and centrifuged at 50,000 ×g at 4°C for 20 min to remove HbV particles. The supernatant was stored at -80℃ until analysis. Plasma NOx levels were measured at the Eicom Laboratory (Eicom Corporation, Kyoto, Japan) using a high-performance liquid chromatography-Griess system, as previously described22.