Title: Identification of the metabolites of ivermectin in
humans
Running title: Identification of ivermectin metabolites
Phornpimon Tipthara1, Kevin C
Kobylinski2, Markus Godejohann3,
Borimas Hanboonkunupakarn1,4, Alison
Roth5,6, John H Adams5, Nicholas J.
White1,7, Podjanee Jittmala1,8,
Nicholas P.J. Day1,7, Joel
Tarning1,7
1Mahidol Oxford Tropical Medicine Research Unit,
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
2Department of Entomology, Armed Forces Research
Institute of Medical Sciences, Bangkok, Thailand.
3AIC, Bruker BioSpin GmbH, Rheinstetten, Germany.
4Department of Clinical Tropical Medicine, Faculty of
Tropical Medicine, Mahidol University, Bangkok, Thailand.
5Center for Global Health and Infectious Diseases
Research, College of Public Health, University of South Florida, USA.
6Department of Drug Discovery, Experimental
Therapeutics Branch, Walter Reed Army Institute of Research, Silver
Spring, MD, USA.
7Centre for Tropical Medicine and Global Health,
Nuffield Department of Clinical Medicine, University of Oxford, Oxford,
United Kingdom.
8Department of Tropical Hygiene, Faculty of Tropical
Medicine, Mahidol University, Bangkok, Thailand.
Word count : 4,549
Acknowledgements: We thank Markus Winterberg and Siribha Apinan
for their contribution to the MS experiments. We are grateful to the
volunteers for their participation in the trial, the nurses of the
Clinical Therapeutics Unit and the Mahidol University Hospital for
Tropical Diseases for their support conducting the trial. This study was
funded through a grant from the Bill & Melinda Gates Foundation
(OPP1180249). The Mahidol Oxford Tropical Medicine Research Unit is
funded by the Wellcome Trust of Great Britain.
Disclaimer: Material has been reviewed by the Walter Reed Army
Institute of Research. There is no objection to its presentation and/or
publication. The opinions or assertions contained herein are the private
views of the authors, and are not to be construed as official, or as
reflecting true views of the Department of the Army or the Department of
Defense. The investigators have adhered to the policies for protection
of human subjects as prescribed in AR 70–25.
Conflict of interest statement: The authors declare no
conflicts of interest. We did not purchase any of the compounds or
instruments mentioned in this article from Bruker.
Correspondence: Professor Joel Tarning, Mahidol Oxford Tropical
Medicine Research Unit, Faculty of Tropical Medicine, Mahidol
University, 420/6 Rajvithi Road, Bangkok 10400, THAILAND. Email:
Joel@tropmedres.ac
Authorship contributions: P.T., K.C.K. and J.T. designed the
research. P.T. performed MS experiments. P.T. analysed data and wrote
the first draft of the manuscript. M.G. performed NMR experiments. P.J.
and B.H. performed the clinical trial. A.R. and J.H.A. performed the
hepatocytes experiments. All authors contributed to the organization of
the study and the final draft of the manuscript.