Inflammatory cytokines and cAMP assay
Plasma in vivo models and cell culture media supernatants from treated macrophages were used to assess the concentrations of inflammatory cytokines (IL-1β, IL-6, TNFα, IL-10) and cAMP by Enzyme-linked immunosorbent assay (ELISA). IL-1β (ab197742; Abcam), IL-6 (ab222503; Abcam), TNFα (ab208348; Abcam), IL-10 (ab255729; Abcam) and cAMP (JL13362; Jianglai Bio, China) ELISA kits were used as per the manufacturer’s instructions.