Cell culture
Myoblasts were isolated from the biopsied tissue by muscle explants and
then purified by CD56 magnetic separation. Early passage myoblasts were
grown until 60-70% confluent on 0.1% gelatin-coated flasks in
proliferation medium containing DMEM supplemented with 10% FBS, 22%
M-199, PSF 1x, insulin 1.74 μM, L-glutamine 2 mM, FGF 1.39 nM and EGF
0.135 mM. Cells for DNA extraction and FISH staining were obtained
between passages P2 and P6.