Figure legends
Figure 1 : RNA foci staining in myoblasts both on coverslips and
in C1 IFC chip. A ) Myoblasts stained in suspension and
visualized on a coverslip with fluorescence (standard
immunocytochemistry), where nuclei are stained in blue and RNA foci in
red. B ) Myoblast stained in suspension, captured in the chip
and visualized with the brightfield channel, in which a myoblast can be
observed. C ) Myoblasts stained in suspension, captured in the
chip and visualized with fluorescence. Note that DAPI (blue) can be
observed but not RNA foci (red).
Figure 2 : RNA foci heterogeneity in the cells of one DM1
patient (n = number of foci). Some of the foci that are more difficult
to see in these images are indicated by white triangles. Foci staining
in green and nuclei in blue.
Figure 3 . Distribution of RNA foci per myoblast in every
patient of this study. 0 = myoblasts with no RNA foci, 1 = myoblasts
with 1 RNA foci, 2= myoblasts with 2 RNA foci, etc.
Figure 4 : Column graph of single cell DMPK (n = 245 for
controls and n = 130 for patients) and exon 7 exclusion MBNL1 (n=
232 for controls and 147 for patients) splicing variant expression
levels between patients and controls. Bars indicate standard deviation.
Figure 5 : Grouped scatter plots for DMPK andMBNL1 exon 7 exclusion isoform single cell data in patients and
controls of our study. Bars indicate standard deviation.