3.1 Production of pseudotyped EBOV
A diagram of pseudotyped EBOV production is shown in Fig. 1c. Briefly, the two-plasmid system (2 μg of each of plasmids) was transfected into HEK293T cells in a 6-well plate, pseudotyped EBOV-containing supernatants were harvested at 24-36 h-post-transfection, followed by titer determination.