2.2 Recombinant plasmid construction and generation of EBOV
pseudotyped virus
The complete genome of EBOV is shown in Fig. 1a, and the full-length G
gene of EBOV (GenBank accession no. KJ660346.2) was synthesized by
Sangon Biotech Biotechnology Co., Ltd. (Shanghai, China) and cloned into
the pcDNA4.0 vector. The recombinant plasmid was confirmed by enzyme
digestion analysis and by DNA sequencing, and the details of the
recombinant plasmid are shown in Fig. 1b. The pseudotyped EBOVs was
produced by the transfection of HEK293T cells with a two-plasmid system,
which consists of the GP-encoding plasmid (pcDNA4.0-G) and
luciferase-expressing plasmid (pNL4-3.luc.RE).