3.1 Production of pseudotyped EBOV
A diagram of pseudotyped EBOV production is shown in Fig. 1c. Briefly,
the two-plasmid system (2 μg of each of plasmids) was transfected into
HEK293T cells in a 6-well plate, pseudotyped EBOV-containing
supernatants were harvested at 24-36 h-post-transfection, followed by
titer determination.