2.5 Microscopy
After staining with 1% sodium phosphotungstate, the grids loaded with EBOV pseudotyped virus were observed using transmission electron microscopy (TEM). For immunoelectron microscopy (IEM), the EBOV pseudotyped virus was also bound to formvar-coated grids, and then incubated with murine anti-EBOV GP monoclonal antibody and gold-labeled goat anti-mouse IgG (Sigma-Aldrich, Saint Louis, MO, USA). After, the formvar-coated grids were stained with 1% sodium phosphotungstate and observed.