2.5 Microscopy
After staining with 1% sodium phosphotungstate, the grids loaded with
EBOV pseudotyped virus were observed using transmission electron
microscopy (TEM). For immunoelectron microscopy (IEM), the EBOV
pseudotyped virus was also bound to formvar-coated grids, and then
incubated with murine anti-EBOV GP monoclonal antibody and gold-labeled
goat anti-mouse IgG (Sigma-Aldrich, Saint Louis, MO, USA). After, the
formvar-coated grids were stained with 1% sodium phosphotungstate and
observed.