Figure 2. Decay curves of Japanese jack mackerel eDNA for (a) mtS (164 bp fragment of CytB gene), (b) mtL (682 bp fragment of CytB gene), (c) nuS (164 bp fragment of ITS1 region), and (d) nuL (603 bp fragment of ITS1 region) observed in the tank experiment. Concentrations of target eDNA (log-transformed) in sampling tanks with and without BAC addition are shown as circles and triangles respectively. Breakpoints between different phases of degradations are shown as dotted lines, which were estimated by the package ‘segmented’ in R.