Takahara et al. (2015) |
Water |
・Freezing (-30 °C) |
1000 mL |
Detection rate of common carp (Cyprinus carpio) eDNA was lower in
frozen samples than in non-frozen samples, while eDNA concentrations
were not different between treatments. |
Williams et al. (2016)
|
Water
|
・Longmire’s solution1
(1: 3 of preservative to water)
・freezing (-80 °C)
|
15 mL
|
Detectability of wild pig (Sus scrofa) eDNA did not decrease in
both treatments for 58 days.
|
Doi et al. (2017)
|
Water
|
・Isopropanol precipitation
(1: 1 of preservative to water)
・Ethanol precipitation
(1: 2 of preservative to water)
|
27 mL (Isopropanol)
15 mL (Ethanol)
|
Yields of common carp eDNA were not different between treatments.
|
Yamanaka et al. (2017)
|
Water
|
・BAC2 solution
(0.01 % of preservative to water)
|
500 mL
|
92 and 50 % of bluegill sunfish (Lepomis macrochirus) eDNA
retained after 8 hours and 10 days at ambient temperature.
|
Ladell et al. (2019)
|
Water
|
・Ethanol precipitation
(1: 2 of preservative to water)
・cooling (4 °C)
|
15 mL
|
Silver carp (Hypophthalmichthys molitrix) eDNA was amplified from
the samples preserved by ethanol precipitation after up to 7 days even
at room temperature.
|
Sales et al. (2019)
|
Water
|
・BAC2 solution
(0.01 % of preservative to water)
・cooling
|
1000 mL
|
MOTUs were higher in the samples stored in ice than those preserved in
BAC at ambient temperature.
|
Takahara et al. (2020)
|
Water
|
・BAC2 solution
(0.01 % of preservative to water)
・cooling (4 °C)
・freezing (-25 °C)
|
500 mL
|
Preservative effect of BAC on eDNA degradation was similar among species
but not among storage temperature.
|
Renshaw et al. (2015)
|
Disk filter
|
・Longmire’s buffer1
・CTAB buffer3
|
250 mL
|
Bluegill sunfish eDNA concentrations did not decrease in both treatments
for 2 weeks and was higher in the filter in Longmire’s buffer.
|
Spens et al. (2017)
|
Enclosed filter
|
・Freezing (-20 °C)
・Ethanol
・Longmire’s buffer1
・RNAlater
|
15 mL
|
European perch (Perca fluviatilis) eDNA yields were higher in the
filter in ethanol or Longmire’s buffer.
|