Figure 5. (a) Comparisons of species richness inferred by eDNA metabarcoding between BAC treatments for each sampling time point. Each Venn diagram shows the differences of the number of fish species detected by eDNA metabarcoding between BAC treatments. Each sampling triplicate is pooled here. (b) A two-dimensional nMDS plot based on Jaccard dissimilarity among BAC treatments and time points. Each symbol represents BAC treatments (Y: with BAC addition; N: without BAC addition) and time points (0, 6, or 24). Plots based on each triplicate in the same treatment are encircled by triangles.