Supporting Information
Appendix S1. Information on primers/probe development.
Appendix S2. Information on data preprocessing and analyses of iSeq raw reads.
Figure S1. Rarefaction curves in eDNA metabarcoding using seawater samples.
Table S1. Detailed information on water sampling.
Table S2. Details of sequence information from the National Center for Biotechnology Information (NCBI) for the development of primers and probe.
Table S3. Results of linear models for field samplings.
Table S4. R2 values, slopes, and Y intercepts of the calibration curves, and the PCR efficiencies (mean ± 1 SD) for each type of eDNA.
Table S5. Detailed list of fish species detected from seawater samples by eDNA metabarcoding.
Table S6. Detailed information on fish eDNA metabarcoding using seawater samples.