Literature Preserved sample Method Water volume per sample Major findings
Takahara et al. (2015) Water ・Freezing (-30 °C) 1000 mL Detection rate of common carp (Cyprinus carpio) eDNA was lower in frozen samples than in non-frozen samples, while eDNA concentrations were not different between treatments.
Williams et al. (2016)
Water
・Longmire’s solution1 (1: 3 of preservative to water) ・freezing (-80 °C)
15 mL
Detectability of wild pig (Sus scrofa) eDNA did not decrease in both treatments for 58 days.
Doi et al. (2017)
Water
・Isopropanol precipitation (1: 1 of preservative to water) ・Ethanol precipitation (1: 2 of preservative to water) 27 mL (Isopropanol) 15 mL (Ethanol)
Yields of common carp eDNA were not different between treatments.
Yamanaka et al. (2017)
Water
・BAC2 solution (0.01 % of preservative to water)
500 mL
92 and 50 % of bluegill sunfish (Lepomis macrochirus) eDNA retained after 8 hours and 10 days at ambient temperature.
Ladell et al. (2019)
Water
・Ethanol precipitation (1: 2 of preservative to water) ・cooling (4 °C)
15 mL
Silver carp (Hypophthalmichthys molitrix) eDNA was amplified from the samples preserved by ethanol precipitation after up to 7 days even at room temperature.
Sales et al. (2019)
Water
・BAC2 solution (0.01 % of preservative to water) ・cooling
1000 mL
MOTUs were higher in the samples stored in ice than those preserved in BAC at ambient temperature.
Takahara et al. (2020)
Water
・BAC2 solution (0.01 % of preservative to water) ・cooling (4 °C) ・freezing (-25 °C)
500 mL
Preservative effect of BAC on eDNA degradation was similar among species but not among storage temperature.
Renshaw et al. (2015)
Disk filter
・Longmire’s buffer1 ・CTAB buffer3
250 mL
Bluegill sunfish eDNA concentrations did not decrease in both treatments for 2 weeks and was higher in the filter in Longmire’s buffer.
Spens et al. (2017)
Enclosed filter
・Freezing (-20 °C) ・Ethanol ・Longmire’s buffer1 ・RNAlater
15 mL
European perch (Perca fluviatilis) eDNA yields were higher in the filter in ethanol or Longmire’s buffer.