Figures
Figure 1. Overall flowchart of the experiments in this study. We transferred 60-L aliquots of rearing water from a stock tank, where Japanese jack mackerels were kept, into two sampling tanks and added BAC solution to one of them (a). In addition, we collected 18 seawater samples from a floating pier in the MFRS and added BAC solution to nine of them (b). Time-series water sampling and filtration was performed for 96 hours (a) or 24 hours (b) followed by DNA extraction, quantitative real-time PCR, and high-throughput sequencing.