Contents of scaffolds
IR spectra (Fig. 4–7) and elemental analysis (Table 3) of the scaffolds
have been performed to check whether gelatin remained in their
structure. Only scaffolds are having proper morphology and made of PLLA
and PCLA before and after rinsing, and the bath was examined. Since PVP
and Pluronic® are biocompatible, the scaffolds have not been tested for
their presence. For comparison, analyses of raw materials (PLLA, PCLA,
gelatin non-woven) and dried mixtures of raw materials, of which
scaffolds were obtained, have been performed (before the gelling and
rinsing baths).
IR spectra of mixtures of dried raw materials use to prepare scaffolds
(before the gelling and rinsing baths), clearly show a significant
presence of gelatin (Figs. 4, 6: bands within the range 3500–2800
cm-1). In the spectra, bands related to PLLA (1750 and
1180 cm-1) (Fig. 4, 5) and PCLA (1420 and 1290
cm-1) (Fig. 6, 7) respectively, could be observed. In
the spectra of scaffolds after the gelling and rinsing baths (Fig. 5, 7)
only the bands related to PLLA and PCLA are visible. There are no bands
specific for gelatin.