Preparation of the scaffolds using gelatin nano-non-woven
Solutions of polyesters in chloroform were poured on the glass base.
After pouring the layer of the solution, a gelatin nano-non-woven was
placed on it. The nano-non-woven was saturated with the solution of
polyester. The residual air was removed by pressing it with a roller.
Then, another layer of the solution was poured, and the polymer
nano-non-woven was placed on it. Immediately after placing the second
layer of the non-woven, it was pressed against the previous layer. The
scaffold was gelled in methanol at –18 °C for 2 h. After the
coagulation of the polymer, the scaffold was rinsed (MiliQ water, 40 °C,
2 h) to remove the gelatin nano-non-woven. After removing pore
precursors, the scaffold was dried out at a temperature of 40 °C under a
vacuum of 1.5 hPa. Dry scaffolds were stored in 70% ethanol.