Materials
The following polymers were used for obtaining the scaffolds:
poly-L-lactide (PLLA) having M n 86 000 g/mol,
Nature Works NW 2003D and poly-ε-caprolactone (PCL) havingM n 85 000 g/mol,
poly(ε-caprolactone-b -lactide) (PCLA) havingM n 80 000 g/mol that was obtained
in-house. Polymers like polyvinylpyrrolidone (PVP) ofM n 10 000 g/mol and Pluronic® F-127 from Sigma
Aldrich were used as pore precursors. The following solvents were used:
chloroform, methanol, ethanol 99%, (all from POCh SA). Ultrapure water
of the resistance of 18.2 MΏ·cm was obtained using a MiliQ device.
For obtaining polymer nano-non-woven, the type A gelatin from the pork
skin from Sigma Aldrich was used. Acetic acid and formic acid (p.a.)
from Avantor PM Poland were used as solvents.
Jurkat cells were used for examining of cytotoxicity. The cytochemical
reaction was performed with the use of propidium iodide from Sigma
Aldrich.
Mouse fibroblasts from the L929 cell line were used to determine
material toxicity and cell interaction with the scaffold surface. The
Presto blue test determined cell viability.