Cytokine and chemokine response
CCL2, CCL3, IL-8, OSM and LIF were expressed at high levels at baseline
(Table 1). IL-13, IL-8, LIF, HGF, and CCL2 (Figure 2, a-c, e, g) and
membrane bound CSF-1 and FL3L (Figure 3, h, i) were only upregulated
through high affinity IgE activation at all times investigated. OSM,
CCL3 and CCL4 were released by stimulation through both low- and high
affinity IgE activation at 3 hours, but at higher levels for high
affinity IgE (Figure 2, d, h, i). Production through high affinity IgE
activation of CCL3 and CCL4 had ceased at 24 hours. CCL7/MCP3 (Figure 2,
j) was significantly upregulated through low affinity IgE activation.
Cytoplasmic markers in the supernatant
CASP-8 (Figure 2, k) was significantly upregulated with low affinity IgE
and differentially regulated with low- and high affinity IgE activation.
SIRT2 (Figure 2, l) was significantly upregulated with low affinity IgE
but not with high affinity IgE activation. STAMBP (Figure 2, m) was
upregulated through high affinity IgE activation only.