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Figure legend:
Figure 1:
A The consensus analysis process for basophil activation starts with identification of a region containing basophils on a scatter plot, followed by selection of basophils with either two or one basophil specific marker and is completed by setting the threshold at 2.5% of resting basophils (blue), before the fraction of activated basophils (red, 74%) is obtained.
B Data was acquired for preactivated stabilized basophils and for fresh blood. A stippled line indicates the threshold for a positive BAT at 5%. Results with the stabilized cells reflect the efficacy of detecting activation of the same sample and is more focused than that of either heparin or EDTA stabilized blood. The median of standard deviations of each round was 16.8% for the centrally activated, stabilized basophils and 49.2% for the locally activated basophil preparations (p=0.03 Wilcoxon Signed Rank test).