Regulation of carbon fixation
Biological
process enrichment analysis based on DEPs showed no significant
change in the carbon fixation
pathway at the protein level. However,
both Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) and phosphoribulokinase (PRK), involved in
carbon fixation pathway, were
dissociated from the GAPDH/CP12/PRK complex by the downregulated CP12,
indicating the activation of GAPDH and PRK. Moreover, thioredoxin
reductase (NTRC), which is associated with the activation of
thioredoxins-regulated enzymes in
carbon fixation pathway, was
upregulated (Table S1). The good
function of the carbon fixation pathway during photoinhibition was
supported by the significantly increased carboxylase activity
of
ribulose bisphosphate
carboxylase/oxygenase (Rubisco) (Fig. 3A). Further supporting this
function,
photosynthetic
rate characterized by O2 evolution rate exhibited
a typical photoinduction
process followed by a normal level
(Fig. 3A).