3.2. Internalization of EVs by BMSCs
To explore whether EVs could enter the cytoplasm of BMSCs, the six
groups of EVs were labeled with green fluorescence dye PKH67 and
incubated with BMSCs for 48 h. Fluorescence microscopy analysis showed
that the BMSCs were capable of internalizing the EVs of all groups(Figure 3) . The internalized PKH67 fluorescence varied very
little between EV groups and across cells within the culture, which
suggests that the EVs were homogenously internalized.