Figure Captions
Figure 1. Schematic diagram of the experimental design for comparison study.
Figure 2. Characterization of stem cell-derived EVs: (A) Protein concentrations. (B) Lipid concentrations. ( C) Morphological analysis by TEM of EVs derived from PSCs and BMSCs at D0, D7, and D21. (D) DLS analysis of EVs show a similar hydrodynamic diameter around 40-100 nm.
Figure 3. Visualization of EVs in BMSCs. EVs are labeled in green with PKH67 while cell nuclei was stained blue with DAPI.
Figure 4. (A) Altered miRNA profiles of EVs derived from 2 type stem cells and between D0 and D21. (B) Pie chart showing the correlation of the top 44 abundant microRNAs (based on FDR <= 0.05) among EVs derived from both cell types. (C) Heatmap of the most abundant miRNAs depiction expression change between cell types and over time.
Figure 5. ALP activity of BMSCs after incubation with EVs (10 µg/ml) after (A) 7 and (B) 14 days of osteogenic differentiation.
Figure 6. Calcium content assay: (A) Alizarin Red S stained images at 7, 10, and 14 days after osteogenic differenation. (B) Alizarin Rdd quantification at 10, 14, and 21 days after osteogenic differenation.