Figure 3. Mutation of PRDM16 decrease brown adipogenesis. (A ) The mRNA level of PRDM16 . (B) The mRNA level of PPARγ at fully differentiation. (C ) Protein expression of PRDM16 and PPARγ. (D ) mRNA levels of brown fat-selective genes analyzed. (E ) Protein expression of UCP1. Data are means ± SE. (A, B, E) n=5–6/group; (C, F ) n=3/group. One-way ANOVA with Tukey post-hoc test was used to compare groups. *P < 0.05, **P < 0.01, ***P < 0.001.