B cell and DC activation early after immunization
To investigate the early activation of B cells by the immunogen, mouse
lymphocytes from inguinal lymph nodes were analyzed on the
3rd, 6th and 9thdays after the first immunization. As shown in Fig. 4A-B, on the
3rd and 6th days (D3 and D6) after
the first immunization, the immune group showed a variable increase in
the percentage of CD19+ CD40+double-positive cells in the inguinal lymph nodes, with values differing
significantly from those of the control group. In addition, ZI-△-PA-GEM
caused the strongest activation, with greatest significant differences
compared to the control group (Fig. 4A, D3 and D6).
Next, the activation of DCs was analyzed by detecting the proportion of
CD11c+ MHC II+ cells (Fig. 4C-D). On
the 3rd day, there was slight activation of DCs, but
there was no significant difference among the groups. On the
6th and 9th days after the first
immunization, the immune groups had an increased percentage of
CD11c+ MHC II+ double-positive
cells. On the 9th day, the activation levels of some
groups decreased, but the ZI-△-PA-GEM group still showed significant
differences from the control group.