2.5 Photosynthetic pigments content
For the extraction of pigments, fresh leaf samples were homogenized with acetone (>99%) at 4 °C and filtered by Whatman No. 42 filter paper. Finally, the absorbance of extracts was recorded at 662, 645, 495, 480, and 470 nm and pigments contents were calculated using following formulas as µg g-1FW (Dere et al.,1998; Buldaa et al., 2008).
Chlorophyll a= Ca= 11.75A662 - 2.350A645
Chlorophyll b= Cb= 18.61A645 - 3.960A662
Carotenoids= Cx+c= 1000A470 + 2.270Ca - 81.4Cb/227
β-Caroten= Cβ-Car= 17.16A495 - 3.96A480
Lutein= CLut= 11.51A480 - 20.61A495