2.7 Primers
Specific primers were designed using wheat and cucumber gene sequences
for PsbA (Figure S1) and Arabidopsis for PsbS (Figure S2) stored at the
NCBI gene bank (https://www.ncbi.nlm.nih.gov). Primer design was
performed using Primer 3 software, and Beacon Designer; and Snape Gene
software were used to examine the parameters associated with the primers
(Thornton and Basu, 2011). 18S rRNA housekeeping gene was also used as
internal control (Fu et al., 2014). Designed primers were
synthesized by Metabion Company (Germany) with high purity and did not
need purifying (Table 1).