2.7 Primers
Specific primers were designed using wheat and cucumber gene sequences for PsbA (Figure S1) and Arabidopsis for PsbS (Figure S2) stored at the NCBI gene bank (https://www.ncbi.nlm.nih.gov). Primer design was performed using Primer 3 software, and Beacon Designer; and Snape Gene software were used to examine the parameters associated with the primers (Thornton and Basu, 2011). 18S rRNA housekeeping gene was also used as internal control (Fu et al., 2014). Designed primers were synthesized by Metabion Company (Germany) with high purity and did not need purifying (Table 1).