Main genes |
Main genes |
Main genes |
Main genes |
Main genes |
Main genes |
PsbA |
D1
|
39 |
F: AATAGGGAACCGCCGAATAC |
220 |
One
cycle of initial denaturation at 95 °C for 15 min, 45 cycles of
amplification at 95°C for 30 s, 60 °C for 45 s, 72 °C for 45 s, and
ultimately 72 °C for 10 min. |
|
|
|
R: GTATGCGTCCTTGGATTGCT |
|
|
PsbS |
CP22
|
22 |
F: CTCAGCCCAAAGTTCACCAT |
175 |
One
cycle of initial denaturation at 95 °C for 15 min, 45 cycles of
amplification at 95°C for 30 s, 60 °C for 45 s, 72 °C for 45 s, and
ultimately 72 °C for 10 min. |
|
|
|
R: CCACCAGACGTTCCAAAGAT |
|
|
Internal control |
Internal control |
Internal
control |
Internal control |
Internal control |
Internal control |
RID2 |
18S rRNA |
32 |
F: GAGAAACGGCTACCACATCCA |
252 |
One cycle of
initial denaturation at 95 °C for 15 min, 45 cycles of amplification at
95°C for 30 s, 60 °C for 45 s, 72 °C for 45 s, and ultimately 72 °C for
10 min. |
|
|
|
R: CCCAACCCAAGGTCCAACTAC |
|
|