Latent role of RPL26 in regulating respiratory edema of chronic
rhinosinusitis with nasal polyps
Abstract
Backgroud: In order to study how to reduce the edema of CRSwNP, we tried
to co-cultured CRSwNP with glucocorticoid, predicted and verified
possible mechanisms through gene sequencing and CRISPR-Case9 gene
editing technology Method:We co-cultured CRSwNP with glucocorticoid ,
and selected normal CRSwNP as control group. Then analyzed the
differentially expressed genes(DEGs), and the RPL26 gene was found
through bioinformatics analysis. CRISPR-Case9 was used to establish the
RPL26 gene silencing and overexpression cells system; Immunofluorescence
staining and flow cytometry analysis were used to observe changes in
cell morphology and apoptosis. Results: Pearson Correlation to analyze
the correlation of the probe signal values of all specimens and found
that the correlation between the two sets of experimental data was high,
R value was close to 1. A total of 12336 genes were detected, and 5026
genes were differentially expressed. Then screened sequence length of
≤1000, 916 of DEGs were obtained, including 340 up-regulated genes and
576 down-regulated genes. Via bioinformatics analysis, the Ribosome
pathway is most relevant, and the RPL26 plays an important role. The
results of immunofluorescence staining and apoptosis experiments showed
that high expression of RPL26 can effectively reduce the degree of edema
of CRSwNP cells, and has little effect on apoptosis. Conclusion: Highly
expressed RPL26 can effectively reduce the edema of CRSwNP cells,
proving that RPL26 plays an important role in this mechanism, and this
role of RPL26 may be highly correlated with the ribosome signaling
pathway.